Open AccessDifferentially expressed genes during <I>Larix</I> somatic embryo
Author(s) -
Lei Zhang,
Qi Liu,
SangMi Han
Publication year - 2009
Publication title -
yichuan
Language(s) - Uncategorized
Resource type - Journals
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2009.00540
Subject(s) - somatic embryogenesis , suppression subtractive hybridization , biology , somatic cell , embryo , complementary dna , cdna library , callus , cell division , microbiology and biotechnology , gene , genetics , embryogenesis , cell
To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization (SSH). The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis xL. principis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds randomly selected positive clones were sequenced, and 468 UniGenes were obtained finally. According to their function, these ESTs were classified into 19 categories and were involved in many biological process related to plant growth and development such as metabolism, transcription, signal transduction, transport facilitation, cell growth and division, cell structure, cell fate, protein synthesis or degradation, defense etc. Real-time PCR results of several ESTs showed that they were all differentially expressed at the different stages during cell line Y35 somatic embryo maturation.