Open AccessEstablishment and preliminary application of multiplex PCR for detecting the microsatellite markers in silkies
Author(s) -
Xiang-Jun Meng,
Qing Zhu,
Mingya Zhang,
Li Liang,
Yiping Liu
Publication year - 2008
Publication title -
yichuan
Language(s) - English
Resource type - Journals
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2008.00521
Subject(s) - microsatellite , multiplex polymerase chain reaction , multiplex , biology , str multiplex system , polymerase chain reaction , genetics , str analysis , microbiology and biotechnology , gene , allele
In order to obtain the stable PCR combinations for silkies genetic analysis, multiplex PCR was used and its reaction condition was optimized. Five combinations of multiplex PCR with good effects were obtained from eighteen microsatellite markers. Three of them were quadruplex-PCRs, and two were triplex-PCRs. The products of multiplex PCR were further combined into three sets for the electrophoresis on ABI 3100-Avant Genetic Analyzer. The results suggested that the eighteen microsatellite markers could be successfully applied to the silkies genetic analysis.