Open AccessEvaluation of the DPPH Radical Scavenging Activity, Total Phenolic Content and Total Flavanoid Content of Different Solvent Extracts of Catunaregam tomentosa (Blume ex DC) Tirveng Leaves
Author(s) -
Wan Amalina Wan Mamat,
Syed Ahmad Tajudin Tuan Johari,
Muhammad Yusran Abdul Aziz,
Ahmad Syibli Othman,
Abdul Manaf Ali
Publication year - 2021
Publication title -
journal of agrobiotechnology
Language(s) - English
Resource type - Journals
eISSN - 1985-5133
pISSN - 2180-1983
DOI - 10.37231/jab.2021.12.2.248
Subject(s) - dpph , flavonoid , chemistry , ethyl acetate , traditional medicine , dichloromethane , antioxidant , food science , rubiaceae , solvent , botany , chromatography , organic chemistry , biology , medicine
Catunaregam tomentosa (Blume ex DC) Tirveng is commonly known as Khet in Thailand and Bisa Ular or Badang in Malaysia. The tree is widely distributed in the north-east region of Thailand while in Malaysia the tree usually grows in the open waterfront area at Terengganu. This plant belongs to the Rubiaceae family, and the genus catunaregam has interesting pharmacological activities such as anti-inflammatory, antispasmodic, antidysenteric, antifertility and immunomodulatory. In this study, the leaves were extracted using dichloromethane, ethyl acetate and ethanol. Total phenolic was determined by Folin-Ciocalteau method while total flavonoid was determined by the aluminium chloride calorimetric method. Meanwhile, its antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanolic extract was found to have the highest percentages of phenolic and flavonoid content. Interestingly, ethanolic extract also demonstrated strong DPPH scavenging activity with IC50 at 20.07 ± 0.51µg/mL.