Open Accessα-Amylase Inhibition, Antioxidant Activity and Phytochemical Analysis of Calotropis gigantea (L.) Dryand
Author(s) -
Rajendra Gyawali,
Bijay Bhattarai,
S Bajracharya,
Surakshya Bhandari,
Puja Bhetwal,
Kanchan Bogati,
Sumanta Neupane,
Sahana Shrestha,
Ashwinee Kumar Shrestha,
Rajendra Joshi,
Prem Narayan Paudel
Publication year - 2020
Publication title -
journal of health and allied sciences
Language(s) - English
Resource type - Journals
eISSN - 2091-2587
pISSN - 2091-2579
DOI - 10.37107/jhas.143
Subject(s) - chemistry , dpph , ethyl acetate , antioxidant , chromatography , phytochemical , silica gel , chloroform , ascorbic acid , column chromatography , amylase , organic chemistry , biochemistry , food science , enzyme
Antioxidant and α-Amylase inhibitory activity of methanolic extract of Calotropis gigantea (L.) Dryand leaves were evaluated.
Methods: The antioxidant activity was evaluated by DPPH assay. The extract was fractionated in Silica gel loaded column chromatography (CC). All fractions were evaluated for their purity by TLC. Out of 11 fractions from CC, one fraction was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS).
Results: The antioxidant activity of methanolic extract was found satisfactory (IC50268.80 µg/ml) as compared with ascorbic acid (141.82 µg/ml). TLC of a fractions showed a compound at Rf value at 0.45 in toluene: chloroform: methanol with mobile phase ratio 7:2:1 respectively.
Conclusions: Total 17 compounds were identified by GC-MS of ethyl acetate fraction and 5-hydroxyl methyl furfural was major furan compound (59.49%). α-Amylase inhibitory activity of the same fraction showed IC50 value of 0.94 mg/ ml. The Nepalese originated C. gigentea (L.) Dryand possesses antioxidant and α-Amylase inhibitory property.