Open AccessA Novel Report on Killer Yeast Strains Identification Methods
Author(s) -
Najwa Mohammed Jameel Ali Abu-Mejdad,
Adnan Issa Al-Badran,
Abdullah H. Al-Saadoon
Publication year - 2020
Publication title -
maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.144
H-Index - 2
eISSN - 2520-0860
pISSN - 1814-5868
DOI - 10.37077/25200860.2020.32.1.04
Subject(s) - yeast , internal transcribed spacer , biology , identification (biology) , microbiology and biotechnology , botany , ribosomal rna , genetics , gene
This study was conducted to isolate and identify killer yeasts from soil samples that collected from different locations in Basrah and Dhi-Qar provinces. Seventy-five soil samples were collected from different areas, including sandy, arable, surface sediment and uncultivated soil, using dilution methods to cultivate a serial dilution of each soil sample. The results showed that a 112 isolates were identified biochemically using VITEK system and molecularly using internal transcribed spacer (ITS1- 5.8S-ITS2) marker. The molecular identification provided fast and precise identification results for the 112 isolates, whereas the VITEK test resulted low identification efficiency (8.2% were accurate and 91.8 % were not). The Diazonium blue B salts produced a good colour reaction in distinguishing between ascomycetes and basidiomycetes. The PCR was more accurate in identification of killer yeasts compared to the VITEK system.