Molecular identification of Fusarium spp. isolated from tomato plant in Iraq and China
Author(s) -
Mohammed A. Fayyadh,
Adnan Issa AlBadran,
Iman S. Al-Jaafari
Publication year - 2017
Publication title -
basrah journal of agricultural sciences
Language(s) - English
Resource type - Journals
eISSN - 2520-0860
pISSN - 1814-5868
DOI - 10.37077/25200860.2017.23
Subject(s) - fusarium , biology , carbendazim , fungicide , conidium , fusarium solani , fusarium oxysporum , fusarium proliferatum , veterinary medicine , horticulture , botany , medicine
This study was conducted to identify Fusarium spp. isolated from tomato plant in Iraq and China. A total of 12 isolates from Iraq (1-12) and four isolates from China (M1-M4) were used in this study. Based on Morphological characteristics (color, growth pattern, Macro and Micro-conidia shape) high differences between Fusarium isolates were found. Sensitivity test to the fungicide carbendazim revealed that one third of isolates(4/12) from Iraq had EC50 values over than 1000?g/ml indicated that many Iraqi isolates have developed resistance to carbendazim. Based on ITS sequences, Fusarium isolates were identified as follow, isolates 1, 3, 5, 6, 7, 10 were identified as Fusarium oxysporum, isolates 8, 11 as F. solani, isolates 12, M1 and M3 as F. moniliforme, isolates 2, M2 and M4 as F. proliferatum, F. chlamydosporum and F. kyushuense , respectively.
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