Open AccessIdentification Of Cu Resistant Bacteria From Tin Mining-Affected Sea Sediment
Author(s) -
Rahmad Lingga,
Budi Afriyansyah
Publication year - 2020
Language(s) - English
Resource type - Journals
ISSN - 2685-7332
DOI - 10.36987/jpbn.v6i2.1666
Subject(s) - tin , sediment , environmental chemistry , biota , contamination , mangrove , bacteria , metal , environmental science , heavy metals , chemistry , veterinary medicine , biology , metallurgy , ecology , materials science , medicine , paleontology , genetics
Tin mining activity at sea has a various negative influence on the environment. One of them is heavy metal contamination that can affect the life of fisheries biota. This research conducted to isolate and test the Cu heavy metal resistance of marine sediment bacteria that are affected by tin mining activity. Sediment sampling was carried out in the area of tin mining to the mudflat neared to the mangrove area. Bacterial isolation was carried out by spread plate method and bacterial characterization included cell shape, Gram staining and biochemical tests. Furthermore, bacterial isolates tested for resistance to metals with concentrations of 10 ppm, 20 ppm, 40 ppm, 80 ppm and 100 ppm. The results showed that bacterial isolates originating from marine sediments affected by mining activity were resistant to Cu heavy metal at various concentrations. Isolates B6, B8 and A10 showed the highest resistance up to a concentration of 100 ppm