Open AccessDevelopment of primary cell culture from the caudal fin region of snow trout Schizothorax plagiostomus (Heckel)
Author(s) -
Ashish K. Chowdhary,
S. N. Bahuguna
Publication year - 2014
Publication title -
environment conservation journal/environment conservation journal
Language(s) - English
Resource type - Journals
eISSN - 2278-5124
pISSN - 0972-3099
DOI - 10.36953/ecj.2014.151219
Subject(s) - explant culture , biology , trout , primary culture , cell culture , fibroblast , andrology , fetal bovine serum , fish fin , anatomy , in vitro , fishery , fish <actinopterygii> , medicine , genetics
A primary cell culture has been established from the Snow trout Schizothorax plagiostomus. Primary cultures have developed from caudal fin tissues by explant culture technique. Cell outgrowth has been obtained from the caudal fin explant after 5-7 days of explant culture. The culture medium used for the experiment was Leibovitz-15 supplemented with 20% Fetal Bovine Serum. Radiation of cells from the explants started after 2 to 3 days. Both fibroblast like and epithelial like cells were noticed but fibroblast like cells dominated as the growth progressed.