Open AccessCHANGES IN REPRODUCTIVE POTENTIAL, SURVIVAL AND METABOLIC ACTIVITY IN TWO SUCCESSIVE GENERATIONS OF DAPHNIA MAGNA IN THE PIPERONYL BUTOXIDE MEDIUM
Author(s) -
Natasha I. Kolesnikova,
А. О. Морозова,
Darya V. Uskalova,
Elena I. Sarapultseva
Publication year - 2017
Publication title -
toksikologičeskij vestnik
Language(s) - English
Resource type - Journals
ISSN - 0869-7922
DOI - 10.36946/0869-7922-2017-1-52-57
Subject(s) - piperonyl butoxide , daphnia magna , daphnia , biology , cladocera , toxicology , toxicity , antioxidant , branchiopoda , biochemistry , chemistry , zoology , ecology , pesticide , crustacean , organic chemistry
Changes in survival, fertility and metabolic activity in two generations of crustacean Daphnia magna in the piperonyl butoxide medium (PBO) were analyzed in concentrations of 50 to 800 ug/ml in a 21 day experiment in each generation. Metabolism disorders were evaluated using the MTT-assay traditionally applied in vitro to analyze cytotoxicity of medications. The MTT-assay shows in an integral way the amount of oxygen active forms, inactivation of mitochondrial oxidases, ratio of living and dead cells and function of antioxidant enzymes. It was found out that in the first generation, the PBO concentration of 353 ug/l is semi-lethal to Daphnia fertility and to the Daphnia survival it is 650 ug/l. In the second generation the toxic concentration is two-fold lower and amounts to 194 ug/l to fertility and to 200 ug/l to survival. It was revealed that PBO poses the cytotoxic effect on Daphnia.