Open AccessCYTOME ANALYSIS OF EFFECTS OF GENOMIC INSTABILITY INDUCED BY DIFFERENTLY SIZED SILVER NANOPARTICLES IN HUMAN WHOLE BLOOD CULTURE
Author(s) -
Ингель Фаина Исааковна,
Кривцова Елена Константиновна,
Юрцева Надежда Александровна,
Савостикова Ольга Николаевна,
Алексеева Анна Венидиктовна
Publication year - 2017
Publication title -
toksikologičeskij vestnik
Language(s) - English
Resource type - Journals
ISSN - 0869-7922
DOI - 10.36946/0869-7922-2017-1-35-41
Subject(s) - micronucleus test , genome instability , micronucleus , cytochalasin b , mitosis , cytochalasin d , nuclear chemistry , chemistry , clastogen , nanoparticle , biophysics , cytotoxic t cell , aneuploidy , silver nanoparticle , microbiology and biotechnology , biology , cytoskeleton , in vitro , dna , materials science , cell , toxicity , nanotechnology , dna damage , biochemistry , organic chemistry , gene , chromosome
A high bactericidal activity of silver nanoparticles (NHS) suggests the possibility of their use in the preparation of drinking water. On the example of human peripheral blood lymphocytes cultured in micronucleus test with cytochalasin B, effects of genome instability were investigated in a concentration range of 0.005-5.0 mg / l of silver nanoparticles (NHS) with a size of 14.3 ± 0.2 nm (Ag14) and 100.0 ± 11.0 nm (AG100), stabilized with gum acacia and for comparison , those of Ag 2 SO 4 (silver ions). In the same range of concentrations . All substances were suspended or dissolved in drinking water.The results showed that the NPS had genotoxic and cytotoxic effects that made them unsuitable to improve the quality of drinking water. Effects of genome instability determined by the frequency of dividing cells with micronuclei and nucleoplasmic bridges, as well as inhibition of mitotic activity, decrease of proliferative activity and increasing duration of the cell cycle came down in series of Ag 2 SO 4 >> Ag100 >> Ag14. However, frequency rise of asymmetric 3-nucleated cells due to aneuploidy induction was the most characteristic of Ag14 particles.