Open AccessEVALUATION OF NLRP3 INFLAMMASOME PROTEIN EXPRESSION IN ULCERATIVE COLITIS
Author(s) -
Manar Mustafa
Publication year - 2022
Publication title -
wiadomości lekarskie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.133
H-Index - 14
eISSN - 2719-342X
pISSN - 0043-5147
DOI - 10.36740/wlek202203113
Subject(s) - ulcerative colitis , immunohistochemistry , staining , pathology , inflammasome , colitis , medicine , colonoscopy , biology , inflammation , gastroenterology , immunology , disease , colorectal cancer , cancer
The aim: This study aimed to evaluate the NLRP3 immunoreactivity in ulcerative colitis in various clinical presentations.Materials and methods: The retrospective study involved 80 formalin fixed paraffin embedded tissue blocks. These were divided into 50 samples of ulcerative colitis and 30 with normal colonoscopy findings. NLRP3 protein expression patterns were evaluated in various cellular components in tissue sections using immunohistochemistry method.Results: NLRP3 inflammasome was significantly expressed with higher percentage among ulcerative colitis tissue sections compared with normal tissue sections. Intense staining was observed in Paneth cells at the base of crypts, inflammatory cells infiltrated between glands and near the base of crypts. Variable intensities of NLRP3 staining were observed in surface epithelial cells and glandular epithelium. Statically higher percentage of expression was found among active disease and patients with extra-intestinal complications.Conclusions: The NLRP3 protein expression pattern was upregulated among various cellular compartments among ulcerative colitis and correlated with disease activity.