Open AccessAGE-RELATED MORPHO-FUNCTIONAL CHANGES IN RATS’ PANCREAS UNDER HIGH-FAT DIET-INDUCED INSULIN RESISTANCE AND ITS PHARMACOLOGICAL TREATMENT
Author(s) -
Tatyana Kvitnitskaya-Ryzhova,
H. V. Kosiakova,
Sergiy P Lugovskoy,
Sergiy Mykhalskiy,
Pavlo Klymenko,
Svetlana P Malysheva,
Oksana Tkachenko
Publication year - 2021
Publication title -
wiadomości lekarskie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.133
H-Index - 14
eISSN - 2719-342X
pISSN - 0043-5147
DOI - 10.36740/wlek202102112
Subject(s) - medicine , pancreas , endocrinology , insulin resistance , hyperplasia , edema , fibrosis , steatosis , inflammation , insulin
The aim: To determine the set of structural and functional changes in pancreatic islets (PI) of obesity-induced insulin resistant (IR) rats of different age (young and old) fed withprolonged (6 month) high-fat diet (HFD) (58% of fat) and further treatment with N-Stearoylethanolamine (NSE), a bioactive N-Acylethanolamine.Materials and methods: Alimentary obesity-induced IR model in rats of two age groups was used to investigate the influence of age and NSE treatment on pancreas morphology(using histological, histochemical and immunohistochemical techniques) and on several biochemical parameters associated with DM onset.Results: The NSE administration normalized pancreas morphology which was more affected in the old IR group; the signs of inflammation, edema, fibrosis and steatosiswere somehow diminished and PI area became significantly increased. The amount of the A-F-positive insulocytes increased and TUNEL-positive – decreased. Compensatoryhyperplasia in the affected pancreas of both age was an important indicator of NSE stimulating effect.Conclusions: Protective effects of NSE on morpho-functional state of pancreas in HFD-induced IR rats of both age are associated not only with its anti-inflammatory, anti-oxidantand anti-dyslipidemic properties but also with activation of PI hyperplasia and β-cells compensatory mechanisms.