Open AccessThe Iinfluence TENS H Current Over Proliferation of Cancer Cells Depending on the Intensity in in vitro Assay
Author(s) -
Marzena Pełczyńska,
Magdalena Milczarek,
Magdalena Maciejewska,
Joanna Wietrzyk
Publication year - 2019
Publication title -
acta balneologica
Language(s) - English
Resource type - Journals
ISSN - 2082-1867
DOI - 10.36740/abal201903110
Subject(s) - in vitro , cancer , cancer cell , cytotoxic t cell , mtt assay , cancer research , medicine , chemistry , microbiology and biotechnology , biology , biochemistry
The knowledge of influence physiotherapeutic method over proliferation process is insufficient. It is considered that electrotherapeutic methods could accelerate neoplastic process. Thus using this methods are very limited in treatment patients with cancer anamnesis. Unfortunately, there are in literature not a lot of results of base researches in this area. It is much more difficult to exclude possibility proneoplastic activity of this methods than confirm this action.Aim: of the study is checking the influence of TENS current over proliferation of cancer cells in in vitro assay.Materials and Methods: The following human cell lines were used- A549, ES-2, HT29, MCF-7. The cells were plated twenty-four hours before treatment. Then the cells were exposured to TENS H current (0,1 mV and 1mV), t imp.100μs; f 100Hz, constant; during 20 min. The in vitro cytotoxic effects were examined after 96h in SRB assay. In the other experiment there were three- times expositions – 24, 48 and 72 hours after the plated.Results: In this assay it wasn’t observed the acceleration of proliferation of cancer cells after single dose or triple dose expositions to TENS Hcurrent.Conclusion: The TENS H current after repeated doses didn’t accelerate proliferation of cancer cells in in vitro conditions.