Open AccessThe role of mutations in the genes FLG and GSTM1, and cytogenetic aberrations in the developmentof allergic occupational dermatitis in the oilfield workers of the north of Siberia
Author(s) -
Н. Н. Ильинских,
Б. В. Шилов,
И Н Ильинских,
Е Н Ильинских,
А. М. Субботин,
N. N. Ilyinskikh,
Б. В. Шилов,
I. N. Ilyinskikh,
E. N. Ilyinskikh,
А. М. Субботин
Publication year - 2011
Publication title -
rossijskij allergologičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2686-682X
pISSN - 1810-8830
DOI - 10.36691/rja846
Subject(s) - allele , occupational exposure , gene , allergy , genotype , allergic dermatitis , dermatology , medicine , immunology , genetics , biology , environmental health
Background. The objective of the investigation was to determine frequencies of micronucleated cells in thebuccal epithelium in relation to polymorphism of genes FLG and GSTM1 among oilfield workers suffered fromallergic occupational dermatitis.Methods. There were 209 male oilfield workers examined with cases of allergic professional dermatitis at the ageof 21-39 years old. as a control group there were used 207 male workers without allergic occupational dermatitis.we used genealogical method, micronucleous test and polymerase chain reaction to detect restriction fragmentsof genes GSTM1 and FLG.Results. it was detected that oilfield workers with genotypes contented homozygous mutated alleles GSTM1 (0/0)gene showed the higher frequency of micronucleated epithelial cells. it was not detected for FLG gene allelevariants. among the examined oilfield workers with allergic occupational dermatitis there was nobody that wouldhave both mutated alleles of FLG (282de14/282de14) and homozygous GSTM1 (0/0) genes, while in the controlgroup we found some individuals of this type.Conclusions. The significantly higher levels of cytogenetic aberrations in the oilfield workers suffered from allergicoccupational dermatitis are the result of combined effects of various factors on the genome. The investigationcan provide the basis for the elaboration of new scientific criteria for hiring staff in oilfields of west siberia.