Open AccessUSE OF MOLECULAR GENETIC METHODS IN THE STUDY OF HEREDITARY PREDISPOSITION TO ATOPIC DISEASES INCHILDREN
Author(s) -
E. S. Tyumentseva,
Н. В. Петрова,
И.И. Балаболкин,
Vsevolod Pinelis,
E S Tumentseva,
Н. В. Петрова,
И.И. Балаболкин,
Vsevolod Pinelis
Publication year - 2011
Publication title -
rossijskij allergologičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2686-682X
pISSN - 1810-8830
DOI - 10.36691/rja798
Subject(s) - gene , allele , genotype , disease , multifactor dimensionality reduction , genetics , polymorphism (computer science) , candidate gene , genetic predisposition , medicine , biology , single nucleotide polymorphism
Background. Study of the аssociations of susceptibility genes to the development of atopic diseases in children.Materials and methods. All 325 examined children reside on the territory of the European part of Russia whoby according to surveys, Russian by nationality. Analysis of polymorphism in genes of receptors ADRB2, GRL,ALOX5, genes of biotransformation - CYP1A1, CYP2C9, CYP2C19, GSTT1, GSTM1, NAT2 as well as the variants ofthe genes MTHFR and TNFA was performed in patients suffering from atopic disease and in healthy individuals.Using Multifactor Dimentionality Reduction method (MDR) it was defined the most significant model of genegeneinteraction for the development of atopic diseaseResults. Association of the development of atopic diseases with polymorphic variants of the genes: ALOX5 (VNTR)GRL (1220A > G) TNFA (-308G > A) CYP1A1 (6235T > C) and GSTM1 was identified in surveyed children. The highriskalleles and genotypes of developing atopic diseases in pediatric patients were determined. Using MultifactorDimentionality Reduction method (MDR) it was defined the most significant model of gene-gene interaction forthe development of atopic disease, including ADRB2 (79 C >G), (46A > G), CYP2C19 (G681A) was defined.Conclusion. There were identified polymorphic variants of genes and important gene-gene interactions associatedwith development of atopic diseases in children.