Open AccessRegulat ory T-cells in periphera l blood of pat ientswith br onchial ast hma
Author(s) -
E E Kremer,
Н. А. Кириллова,
N V Teplova,
I A Deev,
I V Petrova,
Л. М. Огородова
Publication year - 2011
Publication title -
rossijskij allergologičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2686-682X
pISSN - 1810-8830
DOI - 10.36691/rja781
Subject(s) - foxp3 , peripheral blood , flow cytometry , immunology , asthma , medicine , peripheral , inflammation , gastroenterology , immune system
Background. To investigate the expression of surface molecules CD4+CD25high and CD4+FoxP3+ on regulatoryT-cells (Treg) in patients with different severity of bronchial asthma (BA).Methods. We included 19 patients with severe BA, 12 patients with mild BA and 17 healthy donors. The circulatingpercentage of CD4+CD25high and CD4+FoxP3+ T-reg in peripheral blood was estimated by the flow cytometryanalysis (FACSCalibur, Becton Dickinson, USA).Results. Numbers of peripheral blood CD4+CD25high was significantly decreased in mild BA compared to healthycontrol. Patients with severe BA had increased levels of CD4+FoxP3+ and CD4+CD25high compared to mild BA.Conclusion. Our results demonstrate new scientific data on the phenotypic characteristics and the level of T-regin peripheral blood at different clinical forms of asthma. This fact confirms the existence of immunoregulatorycontrol mechanisms of allergic inflammation involving T-reg.