Open AccessLocal and systemic immune response in patients withsevere atopic dermatitis
Author(s) -
Т М Филимонова,
Т М Филимонова,
O G Elisyutina,
Елисютина Ольга Гурьевна,
Е С Феденко,
Е С Феденко,
D D Niyazov,
Д Д Ниязов,
М Н Болдырева,
М Н Болдырева,
O V Burmenskaya,
О. В. Бурменская,
O. Yu. Rebrova,
О. Ю. Реброва,
Т М Филимонова,
O G Elisyutina,
O G Elisyutina,
Е С Феденко,
D D Niazov,
D D Niazov,
М Н Болдырева,
O V Burmenskaya,
Rebrova O Yu
Publication year - 2011
Publication title -
rossijskij allergologičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2686-682X
pISSN - 1810-8830
DOI - 10.36691/rja771
Subject(s) - atopic dermatitis , scorad , medicine , immunology , immune system , interleukin 10 , cytokine , interleukin 23 , interleukin , peripheral blood , inflammation , dermatology life quality index , psoriasis
Background. to comparatively investigate cytokine gene expression in the skin and peripheral blood of atopicdermatitis (AD) patients and healthy individuals.Methods. Samples of skin and peripheral blood from 48 severe AD patients SCORAD (Scoring Atopic Dermatitis)78,5 [57; 89], IGA (Investigators Global Assessment) 4,2 [3,9; 4,7]) at the age of 17 to 45 years and 20 healthy donorsaged from 19 to 32 years were analyzed for gene expression of cytokines using real time reverse transcriptionpolymerase chain reaction (RT-PCR).Results. In the skin of patients with AD, a significant increase of the level of gene expression was observed forinterleukin IL2R (interleukin) (р=0,0023), IL5 (р=0,002), IL6 (р=0,0023), IL8 (р=0,01), IL12β (р=0,0023), IL10(р=0,0023), IL23 (р=0,002), IL29 (р=0,0023), and TGFβ (transforming growth factor) (p=0,0023) as compared tohealthy individuals. In contrast, no difference between AD patients and healthy donors was detected with respectto cytokine gene expression in the peripheral blood.Conclusions. Activity of IL-2R, IL-8, IL-12β, IL-23, IL-29, and TGFβ that are markers of chronic inflammationand Th1 immune response in severe AD and IL-5, IL-10 that are anti-inflammatory cytokines and markers ofTh2 response was predominant in the skin but not in the blood of AD patients.