Open AccessPolymorphic variants of matrix metalloproteinases genes in children with atopic dermatitis
Author(s) -
О. Е. Семерник,
Семерник Ольга Евгеньевна,
А. А. Лебеденко,
Лебеденко Александр Анатольевич,
Т. П. Шкурат,
Шкурат Татьяна Павловна,
Е. В. Машкина,
Машкина Елена Владимировна,
T K Dreyzina,
Дрейзина Татьяна Константиновна
Publication year - 2019
Publication title -
rossijskij allergologičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2686-682X
pISSN - 1810-8830
DOI - 10.36691/rja1195
Subject(s) - genotype , allele , atopic dermatitis , gene , pathogenesis , gene polymorphism , polymorphism (computer science) , genetics , matrix metalloproteinase , biology , medicine , immunology
Matrix metalloproteinases (MMP) play a special role in the pathogenesis of atopic dermatitis (AD). Therefore, the study of the features of genes responsible for the synthesis of MMP in children with AD is of great scientific and practical interest. Background. To study the role of polymorphic variants of matrix metalloproteinase genes (MMP 9 and MMP20) in the pathogenesis of AD in children. Materials and methods. Allelic variants of320A>C gene MMP20,837T>C gene MMP20, -8202 A>G gene MMP9 in children with AD were studied using the method of allele-specific polymerase chain reaction. The control group consisted of I and IIa the health groups patients of the corresponding sex and age. Results. The results of genetic studies showed that the incidence of alleles and genotypes in the polymorphisms 320A>C of the gene MMP20, 837T>C of the gene MMP20 in patients had no significant differences from the control group (p>0.05). It was established that the A/A-genotype of polymorphism -8202 A>G of the MMP9 gene, prevailed among children suffering from AD at a frequency of 69.2%, whereas in the group of healthy children the frequency of this genotype was 3 times lower (p=0.003). At the same time A/G-genotype (55.7%) was predominant in the control group, while G/G genotype was 2 times lower (21.3%). Thus the risk of AD increased by 7.55 times (OR=7.55 [95% Cl - 2.97-19.21; pG of the MMP9 gene, in particular the risk of developing of skin manifestations of allergy is increased by more than 7 times in A-allele homozygotes.