Thyroid and pituitary-adrenal activity in patients with asthma and endemic subclinical hypothyroidism in response of respiratory tract to a cold stimulus

. In patients with uncontrolled asthma, the stress-induced production of glucocorticosteroids in response to a cold bronchoprovocation test is increased, which is associated with an increase in the level of thyroidstimulating hormone (TSH), characteristic of the syndrome of subclinical hypothyroidism (SH). The peculiarities of thyroid hormone metabolism and changes in pituitary-adrenal homeostasis that develop in asthma in a goiter-endemic region under the influence of cold stress factors on the respiratory system are poorly studied. Aim. To assess the activity of the pituitary-thyroid and pituitary-adrenal systems in response to bronchoprovocation with cold air in asthma patients with cold airway hyperresponsiveness (CAHR) and SH living in conditions of goiter endemic in the Amur Region. Materials and methods . In 34 asthma patients with CAHR, without symptoms of thyroid diseases, the level of disease control was assessed using the Asthma Control Test (ACT) questionnaire. Lung function and airway reaction to a cold stimulus (ΔFEV 1IHCA ) during 3-minute isocapnic hyperventilation with cold (-20ºC) air (IHCA) were measured. Methods of immunological diagnostics in the blood of patients before and after the IHCA test were used to measure the concentrations of thyroid-stimulating (TSH) and adrenocorticotropic (ACTH) hormones, common and active forms of thyroxine (T4) and triiodothyronine (T3), thyroglobulin (TG), autoantibodies to thyroperoxidase (AB-TPO), cortisol. The content of cyclic adenosine monophosphate (cAMP) was determined in leukocytes. Results. The patients were divided into two groups: group 1 included 25 patients with an initially low ( 4, 0) TSH level (4.80±0.46 mU/L, p 0.05), base FEV1 was 95.8±3.3 and 81.0±5.4% (p=0.026), ΔFEV 1IHCA -11.9±0.96 and -13.5±2.7 (p>0.05), respectively. In group 1, in response to IHCA, the level of free T4 increased from 14.2±0.70 to 15.2±0.71 pmol/L (p<0.05), free T3 from 3.73±0.24 to 4.15±0.21 nmol/L (p<0.05). In group 2, a lower level of free T4 was recorded, both before (12.2±1.52 pmol/L) and after IHCA (11.6±1.60 pmol/L, p=0.041). In addition, in group 2 patients, the cortisol concentration at baseline and after IHCA (516.6±31.6 and 397.4±40.4 nmol/L, p<0.05) was significantly lower than in group 1 (628.3±27.5 and 608.3±34.1 nmol/L, p=0.042; p=0.002, respectively). A close correlation was found between ΔFEV 1 and the T4 level before (r=0.54; p<0.01) and after the test (r=0.41; p<0.05), with the amount of cAMP up to (r=0.58; p=0.008) and after the test (r=0.47; p=0.009), as well as the correlation between ΔМEF 50 and the initial level of cortisol in the blood serum (r=0.50; p=0.002). Conclusion . In asthma patients with CAHR living in the region of cold climate and goiter endemic, the presence of SH can be considered as a prognostic sign of the development of dysadaptation of the respiratory system to cold exposure.