Open AccessCurrent opportunities and prospects of confocal laser scanning microscopy in morphological research (review)
Author(s) -
Н. Н. Дорофиенко,
Irina Andrievskaya,
О. A. Udovichenko
Publication year - 2021
Publication title -
bûlletenʹ fiziologii i patologii dyhaniâ
Language(s) - English
Resource type - Journals
ISSN - 1998-5029
DOI - 10.36604/1998-5029-2021-81-135-143
Subject(s) - confocal laser scanning microscopy , confocal , confocal microscopy , context (archaeology) , laser scanning , microscopy , data science , computer science , nanotechnology , biology , pathology , laser , materials science , optics , medicine , microbiology and biotechnology , biophysics , physics , paleontology
. The review discloses the results of scientific works related to a modern high-tech method for conducting morphological studies in biology and medicine – confocal laser scanning microscopy. This method, in combination with immunofluorescence histochemistry, can be used in a variety of studies: from rapid visualization of dynamic processes in living cells to thorough morphological analysis of tissues, spatial distribution of macromolecules in fixed or living cells, automatic collection of three-dimensional data, visualization of several labeled samples and measurement of physiological processes in living cells and tissues of organs. Aim. To determine the current possibilities and prospects of confocal laser scanning microscopy in morphological studies. Results. When analyzing scientific literature data, the opportunities and prospects of using confocal laser scanning microscopy in biomedical and morphological studies are presented. The use of the confocal diagnostic method in gynecology, molecular biology, endocrinology, endoscopy is shown. Particular attention is paid to the application of this research method in embryology. In addition, information about the role of confocal microscopy in the study of microbial pathogenesis in a three-dimensional context is provided. Data on the history, basic principles, technical innovations and advantages of confocal laser scanning microscopy are presented. Conclusion. The study of modern scientific literature has shown the importance of using confocal laser scanning microscopy in modern scientific research and diagnosis of diseases in a clinical setting, which will allow to take a new look at some aspects in modern morphology and medicine.