Open AccessDNA damage by oxidized fatty acids detected by DNA/SPE biosensor
Author(s) -
Ľudmila Sirotová,
Marcela Matulová
Publication year - 2021
Publication title -
nova biotechnologica et chimica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.212
H-Index - 9
eISSN - 1339-004X
pISSN - 1338-6905
DOI - 10.36547/nbc.1307
Subject(s) - chemistry , tbars , dna , biosensor , thiobarbituric acid , biochemistry , linoleic acid , oleic acid , fatty acid , lipid peroxidation , chromatography , organic chemistry , antioxidant
Electrochemical DNA/screen-printed electrode biosensor (DNA/SPE biosensor) was tested for the detection of alterations in DNA formed as a consequence of the reaction between DNA and oxidative products of fatty acids. Interaction of DNA with a mixture of products generated during the oxidation of linoleic and oleic acids manifested DNA damage depending on a tested fatty acid and the presence of hydroperoxides and thiobarbituric acid reactive substances (TBARS) determined after the oxidation of fatty acids. A bigger extent of the DNA damage was registered in the case of the interaction with oxidized linoleic acid with the high content of TBARS. The results achieved suggest the possible application of DNA/SPE biosensor in the detection of an interaction between DNA and products of fatty acid oxidation.