Open AccessANTIMICROBIAL RESISTANCE AND MOLECULAR TYPING OF Salmonella spp. ISOLATED FROM POULTRY IN KHARTOUM STATE, SUDAN
Author(s) -
Hassan Hag Elsafi,
Mohamed Amin Mohamed Siddig,
Anne Muckle,
Jaime Martı́nez-Urtaza
Publication year - 2022
Publication title -
bacterial empire
Language(s) - English
Resource type - Journals
ISSN - 2585-9374
DOI - 10.36547/be.363
Subject(s) - microbiology and biotechnology , nalidixic acid , ampicillin , salmonella , pulsed field gel electrophoresis , cefotaxime , amikacin , ceftazidime , streptomycin , sulfamethoxazole , biology , enrofloxacin , trimethoprim , ciprofloxacin , serotype , tetracycline , integron , clavulanic acid , antimicrobial , antibiotic resistance , amoxicillin , antibiotics , bacteria , pseudomonas aeruginosa , genotype , gene , biochemistry , genetics
Salmonella is considered one of the main foodborne pathogens. In this study a total of 38 Salmonella isolates were recovered from 679 (5.6 %) specimens collected within Khartoum State. The specimens included, intestinal contents 11.4% (14/160) egg 7% (7/99), liver 5% (5/100) skin 5% (10/200), feed 0% (0/40%), water 0% (0/100).. Serotyping revealed the presence of eight serovars: Kentucky 11 (28.9%), Stanleyville 8 (21.05%), Virchow 6 (16%), Alachua 5 (13.16%), Blockley 4 (10.53%), Hadar 2 (5.26%), Typhimurium 1 (2.63%) and Havana 1 (2.63%). Antibiotic resistance profile, using disc diffusion method, indicated that all isolates were sensitive to apramycin, chloramphenicol, cefoperazone and cefotaxime. The 38 isolates were found to be resistant to tetracycline (52.6%), nalidixic acid (50.0%), compound sulfonamide (44.7%), sulfamethoxazole-trimethoprim (31.6%), streptomycin (26.3%), gentamycin (15.8%), neomycin (15.8%), furazolidone (7.9%), ampicillin (5.3%), ciprofloxacin (5.3%), amikacin (2.6%), amoxicillin-clavulanic acid (2.6%), ceftazidime (2.6%), and colistin (2.6%) in decreasing order. Only 20 isolates (52.6%) demonstrated multiple drug resistance. Four of the Salmonella isolates were untypable by XbaI Pulsed Field Gel Electrophoresis (PFGE) technique however the 33 typed isolates were differentiated into 22 PFGE patterns. This study aimed to determine the prevalence, antimicrobial resistance and molecular typing of Salmonella spp. form poultry.