Open AccessISOLATION, CHARACTERIZATION AND IDENTIFICATION OF Lactobacillus fermentum EIPW5A ISOLATED FROM WHEY
Author(s) -
Dipanwita Bhattacharjee,
Barun K Bhattacharyya
Publication year - 2021
Publication title -
bacterial empire
Language(s) - English
Resource type - Journals
ISSN - 2585-9374
DOI - 10.36547/be.248
Subject(s) - probiotic , lactobacillus fermentum , biology , 16s ribosomal rna , antimicrobial , microbiology and biotechnology , isolation (microbiology) , bacteria , antibiotics , lactobacillus , lactic acid , lactobacillus plantarum , genetics
The probiotic organisms are now used widely for different clinical indications. In an attempt to isolate a good probiotic strain for therapeutic applications, we have screened several isolates having probiotic attributes. The essential probiotic characters such as lactic acid production, antimicrobial activity, acid and bile tolerance, vitamin B12 production and antibiotic resistance pattern were considered as parameters for screening of probiotic bacteria from its natural habitats. Considering the said probiotic properties the strain EIPW5A was selected for the present study. The organism was identified as Lactobacillus fermentum based on its morphological, biochemical, physiological characters and 16S rRNA gene sequencing results.