Open AccessDifferences in the Expression of miRNA-126 and Interleukin (IL)-13 in Fully Controlled and Not Fully Controlled Asthma Patients
Author(s) -
Yulia Kartina,
Susanthy Djajalaksana,
Iin Noor Chozin,
Harun Al Rasyid
Publication year - 2020
Publication title -
jurnal respirologi indonesia
Language(s) - English
Resource type - Journals
eISSN - 2620-3162
pISSN - 0853-7704
DOI - 10.36497/jri.v40i2.99
Subject(s) - asthma , microrna , interleukin 4 , interleukin 5 , medicine , interleukin 13 , significant difference , outpatient clinic , real time polymerase chain reaction , immunology , interleukin , biology , cytokine , gene , genetics
Backgrounds: Important finding in the last decades in the field of biology and medicine are micro-RNA (miRNA), including miRNA-126 that are involved in the regulation of interleukin-13 (IL-13) and the T helper 2 cell (Th2) response, the key components of the asthmatic response. The aim of this study is to find the difference in the expression of miRNA-126 and IL-13 serum in fully controlled and not fully controlled groups of stable asthma patients and analyze the relationship between them.
Methods: Case-control studies was conducted in 36 stable asthma patients who visited pulmonary outpatient clinic Saiful Anwar Hospital from September 2018 to March 2019. Subjects were divided into 2 groups, each group consisted of 18 fully controlled asthma and 18 not fully controlled asthma patients. The expression of miRNA-126 was measured by the quantitative polymerase chain reaction (q-PCR) and IL-13 using the enzyme-linked immunosorbent assay (ELISA) method.
Results: The expression of miRNA-126 was higher in the control group (3.499±2.99) than case group (2.719±2.73), but the difference was not significant (P=0.273). The IL-13 levels were higher in the case group (17.285±9.37) than control group (11.681±5.22), and the difference was significant (P=0.009). However, there was no relationship between the expression of miRNA-126 and IL-13 levels in both groups.
Conclusions: There were differences in IL-13 levels, but there were no differences in the expression of miRNA-126 in the two groups. There was no relationship between miRNA-126 expression and IL-13 levels in both groups. (J Respir Indo. 2020; 40(1): 24-32)