Open AccessInvestigation of angiogenic factors in obese rats exposed to low oxygen pressure
Author(s) -
Meral Dağ,
Muhittin Yürekli
Publication year - 2020
Publication title -
medical science and discovery
Language(s) - English
Resource type - Journals
ISSN - 2148-6832
DOI - 10.36472/msd.v7i3.360
Subject(s) - adrenomedullin , adipose tissue , white adipose tissue , medicine , endocrinology , obesity , brown adipose tissue , hypoxia (environmental) , matrix metalloproteinase , lung , oxygen , chemistry , receptor , organic chemistry
Objective: Obesity, which is one of the most important health problems of today's people, remains current due to the risks of illness it brings due to the increase rate in the world.
Material and Methods: Male Sprague Dawley rats were used in our study of obesity. Rats were divided into four groups as standard diet/ normal oxygen, standard diet/low oxygen, high-fat diet/normal oxygen and high-fat diet / low oxygen. For the study, a special cage with a low oxygen level of 17-18% was made in a closed system. After achieving the desired 25% weight increase in obese group rats, blood, liver, lung, white adipose tissue and brown adipose tissue were obtained from the rats. In these tissues, adrenomedullin, hypoxic inducible factor 1-α (HIF1-α) and matrix metalloproteinase-II (MMP-II) levels were measured by ELISA.
Results: According to our results, there was a significant increase in adrenomedullin, HIF1-α and MMP-II in white adipose tissue, and adrenomedullin and MMP-II in brown adipose tissue. It was found that the amount of HIF1-α increased significantly in liver and lung tissues.
Conclusion: According to the metabolic status of adipose tissue, it is thought that the effect of adrenomedullin, HIF1-α and MMP-II can increase vascularization of brown adipose tissue and provide energy consumption.