ANALISIS HIDROKUINON PADA BEBERAPA SEDIAAN KRIM MALAM DENGAN METODA SPEKTROFOTOMETRI UV-VIS
Author(s) -
Yulia Rahma
Publication year - 2020
Publication title -
scientia jurnal farmasi dan kesehatan
Language(s) - English
Resource type - Journals
ISSN - 2502-1834
DOI - 10.36434/scientia.v10i2.242
Subject(s) - chemistry , hydroquinone , chromatography , organic chemistry
ABSTRAK Telah dilakukan penelitian analisis kandungan hidrokuinon dalam 5 sampel krim malam. Tujuan penelitian ini untuk mengindentifikasi dan mengukur kadar hidrokuinon dalam beberapa merek krim malam yang dijual secara online. Analisa kualitatif Hidrokuinon menggunakan reaksi warna dan metoda KLT (kromatografi lapis tipis). Kemudian, penentuan hidrokuinon dalam sampel menggunakan Spektrofotonetri UV-VIS. Dari hasil validasi metoda diperoleh persamaan regresi Y = 0,01544x – 0,0244 dengan LOD dan LOQ 1,1211 ppm dan 3,7370 ppm. Uji presisi dengan nilai %SBR kurang dari 2%. Hasil penelitian menunjukkan bahwa dari kelima sampel (1) 2,16%, sampel (2) 2,22%, sampel (3) 1,81%, sampel (4) 1,95% dan sampel (5) 1,80%. Sampel (1) dan (2) mengandung hidrokuinon diatas batas aman yang diperbolehkan oleh BPOM RI yaitu tidak melebihi 2%. Kata Kunci : Kosmetik, Krim Malam, Hidrokuinon, Spektrofotometri Uv Vis ABSTRACT Hydroquinone content analysis reseacrh has been conducted in 5 cream samples night. The purpose of this studyis to identify and measure levels hydroquinone in several night cream brands that are sold online. Hydroquinone qualitative analysis using color reaction and TLC method (thin layer chromatography). Then determine the hydroquinone in the sample using UV VIS Spectrophotometry. From the results of the method validation obtained regression equation Y = 0,01544x-0,0244 with LOD and LOQ 1,1211 ppm and 3,7370 ppm. Precision test with %RSD value less than 2%. Research result shows that out of the five samples (1) 2.16%, sample (2) 2.22%, sample (3) 1.81%, sample (4) 1.95%, and sample (5) 1.80%. Samples (1) and (2) contain hydroquinone above the safe limit allowed by BPOM RI is not more than 2%. Keywords: Cosmetic, Night Cream, Hydroquinone, UV VIS Spectrophotometry
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