Open AccessRESEARCH OF FEED FOR PRODUCTIVE ANIMALS FOR DETERMINATION OF GENERAL MICROBIAL POLLUTION
Author(s) -
I. М. Kushnir,
G. V. Kolodiy,
S. D. Murska,
I. S. Semen,
U. Z. Berbeka
Publication year - 2021
Publication title -
naukovo-tehnìčnij bûletenʹ deržavnogo naukovo-doslìdnogo kontrolʹnogo ìnstitutu veterinarnih preparatìv ta kormovih dobavok ì ìnstitutu bìologìï tvarin
Language(s) - English
Resource type - Journals
eISSN - 2664-5610
pISSN - 2410-9029
DOI - 10.36359/scivp.2021-22-2.26
Subject(s) - enterobacter , microbiology and biotechnology , biology , klebsiella , klebsiella oxytoca , pseudomonas aeruginosa , gentamicin , enterobacter aerogenes , microorganism , escherichia coli , enterobacteriaceae , antibiotics , bacteria , biochemistry , genetics , gene
The article describes the results of microbiological studies of feed for productive animals. The main purpose of this study was to determine the number of mesophilic aerobic and facultative-anaerobic microorganisms, isolation and identification of microorganisms and determine their sensitivity to antibiotics.
In result of the conducted researches it`s established that the general microbial pollution of the investigated samples slightly exceeded indicators of permissible norm, that as a whole made 18 %.
When conducting identification of cultures, the following microorganisms were isolated - Enterobacter spp., Klebsiella spp., Pseudomonas aeruginosa and Escherichia coli. Herewith no enteropathogenic strains of Escherichia coli were detected.
The isolated microorganisms showed different sensitivity to the studied antibiotics. To the oxacillin sensitive were only two isolates of E. coli and P. aeruginosa and one isolate of Enterobacter spp. all other isolated microorganisms were resistant. To the ampicillin resistant were one isolate of P. aeruginosa, two isolates of Enterobacter spp., and two isolates of E. coli.
To the streptomycin, amikacini and gentamicin sensitive were all isolated microorganisms, highly sensitive to streptomycin - Enterobacter spp., amikacin, gentamicin - Enterobacter spp., Klebsiella spp., P. aeruginosa, ofloxacin - E. сoli, P. аeruginosa, Enterobacter spp., Klebsiella spp.
To the ciprofloxacine resistant were one isolate of E. coli and Enterobacter spp. Also it was found that five isolates of P. aeruginosa and three isolates of Enterobacter spp. and E. coli, two - Klebsiella spp. were resistant to cefazolin. Resistant were two isolates of Klebsiella spp. and one isolate of Enterobacter spp. with zones of growth retardation 9 - 9,7 mm.
To the canakamycin, moderately sensitive were isolates of P. aeruginosa, sensitive were isolates of Enterobacter spp., Klebsiella spp. and E. coli.
Regarding polymyxin B, different isolates of P. aeruginosa had different sensitivity to this antibiotic, of which two isolated isolates were moderately sensitive and three isolates were sensitive.
In determining the sensitivity, it was found out that the resistance of isolated isolates of Enterobacter spp., Klebsiella spp., E. coli and P. aeruginosa to oxacillin was 80 %, ampicillin and cefazolin – 20 %, ciprofloxacin – 13.3 %. Among the isolated microorganisms, 20 % were moderately sensitive to ampicillin, 13.3 % to tetracycline, 33.3 % to canakamycin, 53.3 % to rifampicin, and 20 % to polymyxin B.