Open AccessEXPRESS METHOD FOR DETERMINATION OF GENERAL FEED TOXICITY USING BIOLUMINESCENT MICROORGANISMS PHOTOBACTERIUM PHOSPHOREUM
Author(s) -
Олена Володимирівна Курбацька,
О. Л. Оробченко
Publication year - 2021
Publication title -
naukovo-tehnìčnij bûletenʹ deržavnogo naukovo-doslìdnogo kontrolʹnogo ìnstitutu veterinarnih preparatìv ta kormovih dobavok ì ìnstitutu bìologìï tvarin
Language(s) - English
Resource type - Journals
eISSN - 2664-5610
pISSN - 2410-9029
DOI - 10.36359/scivp.2021-22-2.24
Subject(s) - photobacterium phosphoreum , bioluminescence , extraction (chemistry) , toxicity , chemistry , microorganism , chromatography , food science , volume (thermodynamics) , biology , bacteria , biochemistry , physics , organic chemistry , quantum mechanics , genetics
The aim of the work was to develop an express method for determining the general toxicity of feed using bioluminescent microorganisms Photobacterium phosphoreum. The article describes the stages of development and the algorithm for the implementation of the express method. The development of an express method for biotesting feeds using photobacteria as a biosensor was to determine the possibility of Ph. рhosphoreum to provide an adequate assessment in the event of the action of toxicants, to test the preparation of feed samples for research and to establish the optimal exposure to determine the toxicity of the feed: the optimal feed weight was 10.0 g of extractant ethanol with a volume of 20.0 cm3; the method of extraction (vigorous shaking (15-20) min or extraction with periodic stirring for 24 hours, and the exposure before the study – (20-25) min.
The algorithm of the express method for determining the total toxicity of feed using bioluminescent microorganisms Photobacterium phosphoreum is as follows: a sample of feed weighing 10.0 g is crushed, transferred to a glass bottle, filled with 96° ethanol with a volume of 20 cm3 (this volume can be brought up to 50 cm3, so that alcohol completely covered the sample) and extracted with vigorous shaking (15-20) min. or left for 24 hours, then centrifuged at (1.5-2.0) thous. / min 10 min, after which the supernatant liquid is taken and examined on an EMILITE-1003 A luminometer. During testing, 0.02 cm3 of extract is added to the culture liquid in a volume of 1.0 cm3, the exposure time is noted and changes in the luminescence intensity are recorded on the device through (20-25) min. Under the same conditions, 96° ethanol was added as a control. Measurements are carried out sequentially or in pairs of control-experience, or at once all replicates of control samples, and then research ones. To obtain more reliable values, we recommend examining at least 4 replicates of samples (the number of replicates can be increased to 10).