Open AccessDETERMINATION OF EFFECTIVENESS BACILLUS MEGATERIUM FOR SUBCLINICAL COW MASTITIS
Author(s) -
Natalia Grebenik,
Yа. V. Tytukh
Publication year - 2021
Publication title -
naukovo-tehnìčnij bûletenʹ deržavnogo naukovo-doslìdnogo kontrolʹnogo ìnstitutu veterinarnih preparatìv ta kormovih dobavok ì ìnstitutu bìologìï tvarin
Language(s) - English
Resource type - Journals
eISSN - 2664-5610
pISSN - 2410-9029
DOI - 10.36359/scivp.2021-22-2.11
Subject(s) - subclinical infection , mastitis , zoology , herd , medicine , bacillus megaterium , dairy cattle , lactation , veterinary medicine , globulin , biology , pregnancy , genetics , pathology , bacteria
Diagnosis and treatment of mastitis in dairy farms is currently an important task for veterinarians to prevent the spread of the disease among the herd and to prevent the milk entrance from sick animals as a component of marketable produce.
Research and treatment of subclinical mastitis in dairy cows was carried out in LLC "Agrofirma Lan", Kindrativka village, Sumy district, Sumy region. Cows of experimental (D) groups with subclinical mastitis were feed concentrate with the addition of Bacillus megaterium (1٭109 CFU/g) at a dose of 35 grams per animal over a period of 30 days.
The control group of cows (K) was given the routine food for dairy cows. The aim of the study was to investigate the effectiveness of treatment with probiotic strain Bacillus megaterium (1٭109 CFU/g) in subclinical mastitis.
The presence of possible metabolic changes in the body of cows was determined by biochemical parameters in serum samples (n = 15), which were studied spectrophotometrically by conventional methods (SHIMADZU UV-1800, Japan).
At the end of the study period in cows with subclinical mastitis increase in total protein by 36,67 % and globulin by 75,11% was diagnosed. Prior to the start of the study period, cows with subclinical mastitis had increased urea nitrogen levels by 48,87 %, which is higher than the allowable reference values for cows. Also, the level of metabolites in sick cows at the end of the treatment period was lower by AST by 53,69 % and ALT by 51,33 %, compared with the beginning of the study. The results confirm the absence of toxic effects from the probiotic used for treatment. Also, Bacillus megaterium (1٭109 CFU/g) has a therapeutic effect at a dose of 35 grams per animal and reduces inflammation in the udder. Confirmation of these results is the indicator of the somatic cell count (SCC), which is reduced by 70 times at the end of the experiment compared to the beginning, which classifies the probiotic to the "Extra" grade.