Open AccessUltrasonic and Microwave Co-Extraction Optimization of Polysaccharide of Portulaca oleracea L. Technology and its Effect on Ulcerative Colitis
Author(s) -
Yu-Peng Liu,
Xin Qi,
Yixuan Ma,
Li-jun zhou,
Yang Yang,
Shuang Kong,
Meng-zhen Liu,
Rui Zhao,
Gui-yan Jia
Publication year - 2021
Publication title -
sas journal of medicine
Language(s) - English
Resource type - Journals
ISSN - 2454-5112
DOI - 10.36347/sasjm.2021.v07i12.006
Subject(s) - ulcerative colitis , portulaca , extraction (chemistry) , colitis , polysaccharide , therapeutic effect , medicine , chemistry , chromatography , materials science , gastroenterology , pathology , traditional medicine , biochemistry , disease
The extraction conditions of polysaccharides from Portulaca oleracea L.POP were optimized and its effects on the colonic mucosa of mice with ulcerative colitis were studied. In this study, POP were extracted by ultrasonic and microwave synergism. The extraction process of POP was optimized by a single factor experiment and orthogonal design. HE staining was used to evaluate the pathological changes of colon tissues in mice. The results showed that the optimal extraction conditions were as follows: extraction time was 550 s, microwave power was 40 W, solid-liquid ratio (W/V) was 1:35, and extraction times were 3. Under these conditions, the extraction rate of POP was 3.80%. POP could improve the disease state of UC mice, and the high dose group could significantly reduce the DAI index. The results of the HE experiment showed that POP could reduce inflammatory infiltration and tissue damage of the colon in UC mice. This study provided a theoretical basis for the preparation of POP and its clinical application in the treatment of ulcerative colitis.