Open AccessDiversity and Metagenomic Sequence Analysis of Bacterial Strains Isolated from Different Mangrove Environments in Saudi Arabia
Author(s) -
Seham Sater Alhelaify,
Wael N. Hozzein,
Sulaiman Ali Alharbi
Publication year - 2022
Publication title -
scholars academic journal of biosciences
Language(s) - English
Resource type - Journals
eISSN - 2347-9515
pISSN - 2321-6883
DOI - 10.36347/sajb.2022.v10i03.005
Subject(s) - firmicutes , bacteroidetes , actinobacteria , proteobacteria , planctomycetes , biology , gemmatimonadetes , mangrove , metagenomics , acidobacteria , 16s ribosomal rna , bacterial phyla , microbiology and biotechnology , botany , ecology , bacteria , biochemistry , genetics , gene
Mangroves, which dominate tropical intertidal zones and estuaries, are salt-tolerant plants that reproduce through propagules, which are little reproductive units. Because mangroves can grow in seawater, microorganisms capable of interacting with the host and exerting beneficial effects under salt stress should be present. Three mangrove areas in Saudi Arabia yielded sixteen bacterial and actinobacterial species (Thuwal, Jazan, and Farasan islands). The microbial profile of the examined regions was analysed using a 16S rRNA-based metagenomics technique. Based on 16s rRNA and phylogenetic analysis, the selected isolates were identified. The bacterial extracts were tested for antibacterial activity and pharmacological characteristics. The 16S RNA-based metagenomics revealed that eight phyla of bacteria were found in the same region: Bacteroidetes, Firmicutes, Chloroflexi, Gemmatimonadetes, Proteobacteria, Acidobacteria, Actinobacteria, and Planctomycetes. Bacteroidetes, was the most common phyla, followed by Firmicutes and Proteobacteria. The Bacillus genus was found in the majority of samples, followed by Streptomyces. The pharmacological characteristics of the bacterial extracts were assessed. Antibacterial testing revealed that 13 of 16 bacterial extracts were having antimicrobial activities against at least one of the microorganisms examined.