Open AccessThe Morphology of Lymphatic Endothelial Microchannels in the Renal Cortex of the Mouse
Author(s) -
WeiRen Pan,
Nai-Ying Hu,
Zhian Liu,
Chi Ma,
Zhiwei Liu,
Fei Zhen,
Yuhang Ge,
Rui Ma,
Kai-Xiang Ni,
Fan-Qiang Zeng
Publication year - 2021
Publication title -
scholars academic journal of biosciences
Language(s) - English
Resource type - Journals
eISSN - 2347-9515
pISSN - 2321-6883
DOI - 10.36347/sajb.2021.v09i12.002
Subject(s) - lymphatic system , pathology , lymphatic endothelium , immunofluorescence , confocal , h&e stain , staining , immunohistochemistry , endothelium , microchannel , anatomy , medicine , materials science , antibody , nanotechnology , geometry , mathematics , immunology , endocrinology
Background: The knowledge of the intrarenal lymphatic vasculature, especially the lymphatic endothelial microchannel, still remains unclear, that restricts the development of modern scientific research to support clinical management. Methods: Twelve kidneys harvested from 6 white mice were used for the investigation. Four kidneys were histologically sliced for hematoxylin-eosin, immunohistochemistry and immunofluorescence staining examinations. Four of them were sliced horizontally and coronally into 1mm thick tissues after ventricular perfusion was performed by a solution of 4% paraformaldehyde mixed with Indian ink, and then were inspected by a confocal laser scanning microscope after immunofluorescence staining followed by tissue clearing. Four were sliced and examined by scanning electron microscope. Results: The lymphatic vasculature has been detected in the renal tissue of the mouse. The arrangement of renal lymphatic vessels of the mouse was irregular in the cortex. In the 3-dimensional confocal image, many lymphatic endothelial microchannels were visible in the wall of segmental vessels. The ultra-structural feature of the lymphatic endothelial microchannel was noticed in the SEM image. Conclusion: The intrarenal lymphatic vascularture in the renal cortex of the mouse has been presented and the lymphatic endothelial microchannel has been described in detail. The results will provide basic knowledge of animal experiment for further scientific study and clinical applications.