Open AccessAnti-inflammatory Activity Test of Astaxanthin Extract from Litopenaeus vannamei Shrimp Waste Against the Number of Neutrophils and Lymphocytes in White Rats (Rattus norvegicus) Injected with Carrageenin
Author(s) -
Graciela Carijoan,
Aniek Prasetyaningsih,
Vinsa Cantya Prakasita,
Abner Amadeuz Wisaksono,
Djoko Rahardjo
Publication year - 2021
Publication title -
scholars academic journal of biosciences
Language(s) - English
Resource type - Journals
eISSN - 2347-9515
pISSN - 2321-6883
DOI - 10.36347/sajb.2021.v09i05.002
Subject(s) - astaxanthin , litopenaeus , shrimp , carotenoid , brine shrimp , chemistry , food science , chromatography , biology , pharmacology , fishery
Shrimp is one of the main export commodities in Indonesia which has the potential to have bioactive compounds. Shrimp waste contains carotenoids, one of which is astaxanthin, a red-orange xanthophyll carotenoid which has potential pharmacological effects including anti-inflammatory. The purpose of this study was to test the anti-inflammatory activity of astaxanthin extract from Litopenaeus vannamei shrimp waste against the decrease in the number of neutrophils and lymphocytes in carrageenin-injected white rats. Tests carried out in this study include extraction, qualitative tests using Thin Layer Chromatography (TLC), Salkowski test, quantitative tests using UV-Vis spectrophotometry, High Performance Liquid Chromatography (HPLC), purification using column chromatography, and preclinical tests using edema models of white rat feet with 1% carrageenin injection and various doses of the fraction given were 50 mg / kgBW, 100 mg / kgBW and 150 mg / kgBW, positive control (celexocib 18 mg / kgBW) and a negative control (propylene glycol 1 ml). The relative values of neutrophils and lymphocytes per 100 cells were statistically analyzed using SPSS, with a repeated measure ANOVA for the parametric test and Friedman test for the non-parametric test. The results showed that Litopenaeus vannamei shrimp waste extract contained terpenoids and astaxanthin with concentrations of 0.52 mg/gr crude extract and 220.77 mg/g fraction. The results of parametric and non-parametric analysis (p> 0.05) showed that there was no significant difference between each treatment in the decrease of the relative value of neutrophils and lymphocytes, at 0, 4 and 8 hour. The relative value of neutrophils and lymphocytes was still within the normal limit, which might also indicate that fraction did not change the relative value of neutrophils and lymphocytes which can cause neutrophilia (increased neutrophils) or neutropenia (decreased neutrophils) and lymphocytopenia (decreased lymphocytes) or