Open AccessDetermination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities.
Author(s) -
Dian Anggraini,
Devita Sulistiana,
Dwi Kameluh Agustina,
Almira Ulimaz
Publication year - 2020
Publication title -
jurnal penelitian dan pengkajian ilmu pendidikan
Language(s) - English
Resource type - Journals
ISSN - 2615-6881
DOI - 10.36312/e-saintika.v4i2.170
Subject(s) - chemistry , pectinase , hydrolysis , ion , chromatography , substrate (aquarium) , nuclear chemistry , kinetic energy , kinetics , enzyme , biochemistry , organic chemistry , biology , ecology , physics , quantum mechanics
The purpose of study was to determine kinetic parameters and inhibitory effect of Mg2+ ions on pectinase. Activity test performed at pH 4,5 temperature 55oC for 50' with 10 mm concentrations of each ion 0-10mm. Galacturonic acid content, as product of pectin substrate hydrolysis, was used as basis for determining activity and analyzed by visible spectrophotometry. At concentrations 2 and 4 mm Mg2+ act as an activator, but at concentrations 6, 8, and 10 mM as inhibitors. The value of KM with and without inhibitors is almost same, that is 0.3145% and 0.3105% but value of Vm from both are different, that is 80,645 μg.ml-1.minute-1 and 62,112 μg.ml-1.minute-1. The conclusion that type of inhibitory pectinase with Mg2+ is a non-competitive inhibition. The inhibition constant value (Ki) is 26,84.