Open AccessIN VITRO CLEANING UP AND MICROPROPAGATION OF PERS PECTIVE CHRYSANTHEMUM CULTIVARS AND NEW HYBRID FORMS OF THE NIKITA BOTANICAL GARDENS' BREEDING
Author(s) -
И.В. Митрофанова,
О.В. Митрофанова,
Н.П. Лесникова-Седошенко,
N.V. Smykova
Publication year - 2021
Publication title -
bûlletenʹ gosudarstvennogo nikitskogo botaničeskogo sada
Language(s) - English
Resource type - Journals
ISSN - 0513-1634
DOI - 10.36305/0513-1634-2021-138-92-100
Subject(s) - explant culture , kinetin , micropropagation , cultivar , biology , botany , murashige and skoog medium , horticulture , in vitro , biochemistry
An in vitro micropropagation protocol has been developed for garden chrysanthemum cultivars Mokroe Serebro, Predrassvetnyiy Ayu-Dag, Eldorado, and Ermitage of the NВG's breeding. 10-20 mg/1 of ribavirin promoted the primary explants cleaning up. А high frequency of regeneration of adventitious buds and microshoots was observed in cultivars Eldorado and Ermitage (21.0 and 23.5 microshoots/explant) cultured on MS medium with 0.75 mg/1 kinetin and Mokroe Serebro - on MS medium with 0.75 mg/1 ВАР (24.5 microshoots/explant). Spontaneous root formation facilitated 100% rooting of microshoots.