Open AccessInfluence of the temperature factor on regeneration features of Silene Jailensis N.I. Rubtzov and Crepis Purpurea (Willd.) M. Bieb. and the content of phenolic substances in vitro
Author(s) -
И.В. Митрофанова,
Н Н Иванова,
А. Е. Paliy,
I. N. Paliy,
О.В. Митрофанова
Publication year - 2020
Publication title -
bûlletenʹ gosudarstvennogo nikitskogo botaničeskogo sada
Language(s) - English
Resource type - Journals
ISSN - 0513-1634
DOI - 10.36305/0513-1634-2020-135-87-96
Subject(s) - shoot , botany , regeneration (biology) , caryophyllaceae , biology , in vitro , chemistry , horticulture , biochemistry , microbiology and biotechnology
The results of temperature influence on the regeneration of microshoots and the level of phenolic compounds in microshoots of two rare plant species under in vitro conditions are presented. It is shown that the maximum growth of the main and the formation of adventitious shoots, leaves occurred at a temperature of 21- 23°C. Reconnaissance experiments on the biochemical study of organs and tissues of the studied species were conducted. It was found that the leaves contain high concentrations of phenolic substances and are characterized by a wide variety of components. It is shown that as the temperature increased, the content of the sum of phenolic substances increased, which negatively affected the morphogenetic potential of the studied species.