Open AccessPeculiarities of induction of callusogenesis and morphogenesis in fennel depending on the age of seedlings
Author(s) -
А.Ш. Тевфик,
N.A. Yegorova
Publication year - 2019
Publication title -
bûlletenʹ gosudarstvennogo nikitskogo botaničeskogo sada
Language(s) - English
Resource type - Journals
ISSN - 0513-1634
DOI - 10.36305/0513-1634-2019-133-101-108
Subject(s) - hypocotyl , callus , seedling , explant culture , somatic embryogenesis , biology , botany , cultivar , morphogenesis , regeneration (biology) , horticulture , microbiology and biotechnology , in vitro , biochemistry , gene
The influence of the age of seedlings (of which hypocotyl explants were isolated) and the composition of the culture medium on the callusogenesis and morphogenesis induction of fennel cultivars ‘Mertsishor’ and ‘Oksamit Kryma’ were studied. It was established that with the use of younger seedlings (7-day of age) callus induction began in a larger number of explants for a week of cultivation earlier compared to 14 and 21-day seedlings. The frequency of somatic embryogenesis in the callus obtained from 7-day seedling hypocotyl was almost twice higher than that of the callus from more mature seedling. It was revealed that the cultivar ‘Mertsishor’ had a higher morphogenetic potential compared to ‘Oksamit Kryma’. Improved methods of embryogenic callus induction and plant regeneration can be used for obtaining the initial breeding material of fennel.