Open AccessFirst report of karyological analysis and heteromorphic nucleolar organizer region of Black Surgeonfish (Acanthurus gahhm, Acanthuridae) in Thailand
Author(s) -
Sitthisak Jantarat,
Sarun Jumrusthanasan,
Sarawut Kaewsri,
Praween Supanuam,
Alongklod Tanomtong
Publication year - 2021
Publication title -
caryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.319
H-Index - 29
eISSN - 2165-5391
pISSN - 0008-7114
DOI - 10.36253/caryologia-853
Subject(s) - biology , karyotype , giemsa stain , zoology , nucleolus organizer region , ploidy , nucleolar organizer region , centromere , microchromosome , fishery , genetics , chromosome , gene
This research was the first report on karyological analysis and heteromorphic nucleolar organizer region of black surgeonfish (Acanthurus gahhm, Acanthuridae) in Thailand. The 10 male and 10 female specimens were collected from Phuket Marine Biological Center, and Phang Nga Coastal Research and Development Center, Andaman Sea, Thailand. Mitotic chromosomes were directly prepared from gill and kidney tissues. The chromosomes were stained by conventional Giemsa staining and Ag-NOR banding techniques. Results showed that the diploid chromosomes number of A. gahhm was 2n=48, the fundamental numbers (NF) was 54 in both male and female. The karyotype consist of 6 large acrocentric, 20 large telocentric, 18 medium telocentric and 4 small telocentric chromosomes. None of strange size chromosomes related to sex was found. The heteromorphic nucleolar organizer regions (NORs) were observed on telomeric short arm of first acrocentric which can defined as 1a1b. There is NOR in 1a and not in 1b. The karyotype formula of black surgeon fish was as follows: 2n (48) = La6+Lt20+Mt18+St4