Open AccessInfluence of chronic alcoholic intoxication and lighting regime on karyometric and ploidometric parameters of hepatocytes of rats
Author(s) -
David А. Areshidze,
Yuri Kirillov,
Lyudmila Makartseva,
Maria Kozlova,
И. А. Чернов,
Evgeniya Shtemplevskaya
Publication year - 2021
Publication title -
caryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.319
H-Index - 29
eISSN - 2165-5391
pISSN - 0008-7114
DOI - 10.36253/caryologia-1122
Subject(s) - circadian rhythm , biology , hepatocyte , adaptation (eye) , alcohol , constant (computer programming) , alcohol intoxication , rhythm , physiology , biophysics , medicine , microbiology and biotechnology , toxicology , endocrinology , neuroscience , biochemistry , in vitro , poison control , environmental health , injury prevention , computer science , programming language
The features of the diurnal dynamics of the area of rat hepatocyte nuclei and their ploidy were studied under conditions of a standart (fixed) light regime and constant illumination, as well as under chronic exposure to alcohol in the mentioned light regimes. It has been shown that exposure to alcohol and exposure to constant illumination separately lead to a change in the amplitude-phase characteristics of the circadian rhythm of the nucleus area, while the combined effect of these factors leads to a complete destruction of the rhythm, which indicates a violation of adaptation processes. An increase in the average ploidy of hepatocyte nuclei in chronic alcohol intoxication is also shown, while in animals kept under constant illumination without drinking alcohol, the values of this parameter decrease, which indicates a successful course of the adaptation process. The conducted research indicates that the results of karyometric and ploidometric analysis characterize the degree of influence of alcohol intoxication and changes in the light regime on the liver of rats, reflecting the rate of efficiency of adaptation to these factors.