Open AccessColchicine induced manifestation of abnormal male meiosis and 2n pollen in Trachyspermum ammi (L.) Sprague (Apiaceae)
Author(s) -
Harshita Dwivedi
Publication year - 2021
Publication title -
caryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.319
H-Index - 29
eISSN - 2165-5391
pISSN - 0008-7114
DOI - 10.36253/caryologia-1113
Subject(s) - meiosis , pollen , biology , polyploid , botany , colchicine , metaphase , meiocyte , stamen , chiasma , genetics , ploidy , chromosome , gene
Unreduced gametes are the key source for the natural polyploidization in plants, but process of its formation is very low in nature. Meiotic mutants are second source for the formation of 2n pollen. In this cytological investigation, the meiotic aberrations and its impact on post-meiotic products were analysed in autotetraploid Trachyspermum ammi (L.) Sprague (4n=36). The seedlings of T. ammi (L.) Sprague were treated with 3 different concentrations of colchicine (0.2, 0.4 and 0.5%, w/v) for 3 different durations. Six polyploid plants were induced which was confirmed on the basis of cytological analysis. Colchicine, an anti-microtubular drug induced different meiotic and post-meiotic abnormalities such as chromosomal bridges, lagging chromosomes, scattering, precocious, fragments, dyads, triads, and polyads. The formation of several abnormal sporads clearly signifies the meiotic restitution. The tendency of univalents to scattered in the cytoplasm at metaphase was identified as a peculiar aberration asynapsis. Pollen variability and fusion of pollen walls was reported and pollen fertility was calculated. The morphological analysis of the pollen allowed us to confirm the occurrence of 2n pollen.