Open AccessInvestigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.
Author(s) -
Secil Kivrak Kiran,
Selin Galatalı,
Sevil Yeniocak,
Damla Ekin Ozkaya,
Taner Mercan,
Sevinc Guldag,
Onur Celik,
Naeem Abdul Ghafoor,
Eergun Kaya
Publication year - 2021
Publication title -
advances in horticultural science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.249
H-Index - 24
eISSN - 1592-1573
pISSN - 0394-6169
DOI - 10.36253/ahsc-10536
Subject(s) - shoot , horticulture , cultivar , gibberellic acid , botany , biology , meristem , charcoal , chemistry , germination , organic chemistry
Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.