Open AccessCYTOMEGALOVIRUS DNA CONCENTRATION IN BIOLOGICAL SAMPLES AS A KEY TO THE DIAGNOSIS OF CMV PNEUMONIA IN HIV-INFECTED PATIENTS
Author(s) -
V.I. Shakhgildyan,
M S Yadrikhinskaya,
A. A. Orlovsky,
O. Yu. Shipulina,
Э. А. Домонова,
Е.В. Yarovaya
Publication year - 2020
Language(s) - English
Resource type - Conference proceedings
DOI - 10.36233/978-5-9900432-9-9-86
Subject(s) - bronchoalveolar lavage , pneumonia , cytomegalovirus , sputum , immunosuppression , immunology , medicine , virology , lung , polymerase chain reaction , virus , biology , viral disease , pathology , herpesviridae , tuberculosis , gene , biochemistry
According to examination and follow-up results of 5485 HIV-positive hospitalized patients (3333 of which were diagnosed with AIDS) we have identified the frequency of clinically evident CMV-infection as well as the frequency and character of CMV related lung disease. Statistically significant correlation between viral load, degree of immunosuppression, CMV replication rate and CMV pneumonia development risk has been determined. Qualitative PCR assay for CMV DNA in plasma and respiratory samples was found to have high sensitivity and low specificity for diagnosing CMV-pneumonia. We identified quantitative PCR CMV DNA values in blood cells, plasma, bronchoalveolar lavage, bronchi samples and sputum that confirm the diagnosis of CMV pneumonia with 95% and 99% probability, and exclude CMV related lung damage in HIV patients with 90% and 99% probability.