Open AccessCharacterization of B1-cells during experimental leukomogenesis
Author(s) -
И Ю Ездакова,
О. А. Капустина,
М. И. Гулюкин,
Татьяна Ивановна Степанова
Publication year - 2020
Publication title -
voprosy virusologii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.151
H-Index - 15
eISSN - 2411-2097
pISSN - 0507-4088
DOI - 10.36233/0507-4088-2020-65-1-35-40
Subject(s) - cd5 , bovine leukemia virus , polyclonal antibodies , immune system , antibody , biology , immunology , lymphoblast , immunoperoxidase , virology , monoclonal antibody , cell culture , genetics
Background . Bovine leukemia causes a significant polyclonal expansion of CD5 + , IgM + B lymphocytes, known as persistent lymphocytosis (PL), in approximately 30% of infected cattle. However, it is not yet clear what happens to this subpopulation of B cells in the early period of infection of animals. Purpose . Quantitative characterization of IgM + and CD5 + B cells during the immune response, which can provide important information on the mechanisms of lymphocyte priming in BLV infection. Material and methods . The experiment used BLV-negative calves of black-motley breed at the age of 8 months ( n = 11). Animals ( n = 8) were intravenously injected with blood of a BLV-positive cow. Control calves ( n = 3) were injected with saline. Studies were performed before and after infection on days 5, 7, 14, 21, 28 and 65 of the immune response. The determination of the number of B-lymphocytes in the blood was carried out by the method of immunoperoxidase staining based on monoclonal antibodies to IgM, CD5. Results . As a result of the studies, it was found that the level of CD5 + B cells increases on the 14th day of the primary immune response, characterized by polyclonal proliferation of CD5 + B cells, which are the primary target for BLV. Our research data confirm that in the lymphocytes of experimentally infected cattle, surface aggregation of IgM and CD5 molecules on B-lymphocytes is absent. Discussion . It is known that the wave-like nature of IgM synthesis, which was shown in previous studies, depends on a subpopulation of B1 cells. After 7 days of the immune response, IgM + and CD5 + cells do not correlate, which shows their functional difference. The increase in CD5 + cells is probably not associated with B cells, but with T cells differentiating under the influence of the virus. Conclusions . A subset of B1 cells is the primary target of cattle leukemia virus. The 65th day of the immune response is characterized by the expansion of IgM + B cells, a decrease in the number of CD5 + cells and a uniform distribution of receptors around the perimeter of the cells.