Open AccessManufacturing of hybridomas, producing monoclonal antibodies against <i>Burkholderia mallei</i> and <i>Burkholderia pseudomallei</i> antigens
Author(s) -
Г. В. Куклина,
Г. Д. Елагин,
Д. В. Печенкин,
О. О. Фоменков,
А. В. Еремкин,
А. А. Кытманов,
С. А. Шурупов,
С. С. Ипатов
Publication year - 2019
Publication title -
žurnal mikrobiologii, èpidemiologii i immunobiologii
Language(s) - English
Resource type - Journals
eISSN - 2686-7613
pISSN - 0372-9311
DOI - 10.36233/0372-9311-2019-4-78-82
Subject(s) - burkholderia pseudomallei , monoclonal antibody , antibody , melioidosis , antigen , immune system , microbiology and biotechnology , biology , burkholderia , virology , bacteria , immunology , genetics
Aim. Obtaining hybridomas, stable producing specific monoclonal antibodies against Burkholderia mallei and Burkholderia pseudomallei antigens. Materials and methods. The microbial cultures from State Collection of Microorganisms from the Branch of 48 CSRI of the Defense Ministry of Russian Federation (Kirov) and BALB/c mouse were used in research. Hybridization of B lymphocytes with SP2/0-Ag14 myeloma cells was performed by G.Kohler and C.Milstein procedure in De St. Fazekas and D.Scheidegger modification. The specific activity of immune sera, hybridoma supernatants, ascites and evaluating the diagnostic capabilities of monoclonal antibodies was studied by ELISA. Results. Hybridomas, producing monoclonal antibodies against causative agents of glanders and melioidosis antigens, were obtained and characterized. Obtained hybridomas are active and stable antibody producers after repeated in vitro and in vivo passaging. Immunoglobulins from obtained ascites were isolated. Antibodies provided the greatest sensitivity and specificity were selected. Conclusion. Monoclonal antibodies, producing by obtained hybridomas may be used for creating of immune biological tests.