Open AccessTHE USE OF MASS SPECTROMETRIC ANALYSIS FOR THE DETECTION OF BACTERIAL TOXINS
Author(s) -
М. В. Полеева,
О. С. Чемисова
Publication year - 2018
Publication title -
žurnal mikrobiologii, èpidemiologii i immunobiologii
Language(s) - English
Resource type - Journals
eISSN - 2686-7613
pISSN - 0372-9311
DOI - 10.36233/0372-9311-2018-1-93-101
Subject(s) - bacillus cereus , toxin , microbial toxins , microbiology and biotechnology , shiga toxin , neurotoxin , bacillus anthracis , staphylococcus aureus , escherichia coli , biological warfare , cholera toxin , chemistry , biology , computational biology , bacteria , biochemistry , toxicology , genetics , gene
Toxins - molecular weight compounds produced by microorganisms, animals, plants and possessing antigene properties. Recently due to the perceived threat of terrorist actions identification of a number of bacterial toxins is especially important. A new approach in the identification of toxins associated with the development of mass spectrometry and can be successfully used for analysis of most environmental toxins. The method of MALDI-MS allows the detection of toxins such as Shiga-toxin Escherichia coli, delta-toxin of Staphylococcus aureus, Bacillus cereus particular, botulinum neurotoxin, cholera toxin. Analytical and diagnostic characteristics of the method, the simplicity and speed studies indicate the long term implementation of a method in the practice of laboratory diagnostics in determining toxinproducing of the studied microorganisms.