Open AccessAPPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT
Author(s) -
Д. В. Ульшина,
Dmitry Kovalev,
Д. Г. Пономаренко,
Д. В. Русанова,
Н. М. Швецова,
Т. В. Таран,
И. В. Кузнецова,
А. М. Жиров,
А. А. Хачатурова,
I. Yu. Borzdova,
А. Н. Куличенко
Publication year - 2017
Publication title -
žurnal mikrobiologii, èpidemiologii i immunobiologii
Language(s) - English
Resource type - Journals
eISSN - 2686-7613
pISSN - 0372-9311
DOI - 10.36233/0372-9311-2017-4-9-17
Subject(s) - brucella melitensis , brucella , brucellosis , mass spectrometry , chromatography , brucellaceae , human blood , microbiology and biotechnology , chemistry , biology , virology , physiology
Aim.Study the possibility to apply time-of-flight mass-spectrometry for detection of causative agent of brucellosis in blood. Materials and methods. Brucella strains: 5 Brucella melitensis and 21 Brucella abortus. Protein profiling in linear mode on MALD1-TOF mass-spectrometer Microflex «Bruker Daltonics». Results. Technique for disinfection and preparation of blood samples was modified and optimized for MALDI-TOF MS analysis. 120 representative protein profiles of sera extract were obtained that contain brucellosis causative agent. A resulting peak-list (super-spectrum) of the studied protein fraction of blood extract of a conditionally healthy human within the studied group was formed and analyzed. Conclusion. A scheme of brucella detection in blood samples by MALDI-TOF MS is proposed, based on detection of a complex of 15 genus-specific fragments. Signals on mass-spectra of extracts of leukocyte fraction of blood, artificially contaminated with brucellosis causative agents are characterized.