Open AccessEFFECTIVENESS OF A TEST-SVSTEM FOR DIAGNOSTICS OF MELIOIDOSIS AND GLANDERS BASED ON PASSIVE HEMAGGLUTINATION REACTION AND SOLID-PHASE ENZYME IMMUNOASSAY
Author(s) -
А. А. Будченко
Publication year - 2016
Publication title -
žurnal mikrobiologii, èpidemiologii i immunobiologii
Language(s) - English
Resource type - Journals
eISSN - 2686-7613
pISSN - 0372-9311
DOI - 10.36233/0372-9311-2016-4-86-95
Subject(s) - melioidosis , immunoassay , burkholderia pseudomallei , antigen , microbiology and biotechnology , hemagglutination , virology , antibody , lysis , biology , immunology , bacteria , genetics
Analysis of published results of studies of effect of various antigens of Burkholderia pseudomallei and B. mallei on operation characteristics (sensitivity and specificity) of tests for diagnostics of melioidosis and glanders based on reaction of passive hemagglutination and solid-phase enzyme immunoassay is presented. Methods of isolation of 2 types of antigens are examined: lysate (obtained by lysis of bacterial cells) and recombinant (obtained by genetic engineering, analogues of the determined antigens of the causative agent). Perspectives of production of universal antigens of B. pseudomallei and B. mallei for test-systems based on passive hemagglutination and solid-phase enzyme immunoassay and their application for effective diagnostics of melioidosis and glanders in endemic regions are shown.