Open AccessSpectrum of Aerobic Bacterial Pathogen isolated from Pus samples and their Antibiotic Sensitivity Pattern in a Tertiary Care Hospital
Author(s) -
Soni Soni,
Anita Pandey,
Kalpana Chauhan,
Deepali Saini
Publication year - 2019
Publication title -
international journal of scientific research
Language(s) - English
DOI - 10.36106/ijsr/6700319
Subject(s) - antibiotics , antibiotic resistance , microbiology and biotechnology , antimicrobial , microbiological culture , pathogen , aerobic bacteria , antibiotic sensitivity , gram positive bacteria , gram staining , bacteria , biology , agar diffusion test , medicine , staphylococcus aureus , genetics
Background:Wound infection continues to be a significant problem in clinical practice where empirical treatment of infection is required whilewaiting for culture report to prevent emergence of resistance.Objectives:To study the spectrum of aerobic bacterial pathogen isolated from pus samples and to determine the antimicrobial sensitivity pattern ofthe bacteria isolated.Method: The samples of pusreceived in Clinical Microbiology Laboratory were processed during the study period to isolate and identify bacterialpathogens as per standard bacteriologicaltechniques. Antibiotic susceptibility testing was done by Kirby Bauer disc diffusion method as per CLSIguidelines 2017.Result:Out of the total of 1474pus samples processed, 21.98% samples were culture positive. Majority of culture positive samples were from IPDpatients.Predominance of gram negative bacteria (77.4%) was seen as compared to gram positive bacteria (22.60%) both in IPD and OPD samples.High level of resistance to various antimicrobials was seen among the clinical isolates.Conclusion: Emergence of antibiotic resistant strains in pus sample is a matter of concern. Therefore, knowledge of the spectrum ofmicroorganisms causing pus discharge and its susceptibility pattern is required and this data may contribute to an effective management of cases ofwound infection