Open AccessDETERMINATION OF ANTIMICROBIALACTIVITY USING METHANOLIC EXTRACT OF PITHECELLOBIUM DULCE PLANT LEAVES.
Author(s) -
A Mithaiwala Idris,
S Suthar Hetal,
Hetvi Soni,
Ojha Sunil,
Amit Agarwal
Publication year - 2020
Publication title -
indian journal of applied research
Language(s) - English
DOI - 10.36106/ijar/3204353
Subject(s) - antimicrobial , glycoside , antifungal , traditional medicine , biology , minimum inhibitory concentration , sugar , bacteria , extraction (chemistry) , food science , botany , chemistry , microbiology and biotechnology , chromatography , medicine , genetics
Present days due to the pathogenic bacteria and fungi, the human mortality rate has increased in number. The syntheticantimicrobial and antifungal are used in the treatment of various fungal and bacterial diseases, but have various sideeffects which limit's their use. Traditionally Pithecellobium dulce plant has been used for treatment of antifungal and antimicrobial infection. Inthis article we are comparing the activity of antimicrobial and antifungal properties of Pithecellobium dulce plant using leaves extract on gramnegative bacteria,gram positive bacteria and fungi.Methanolic extraction of Pithecellobium dulce leaves was carried out using Soxhlet methodand the phyto constitutional analysis was carried out for presenceof alkaloids, avonoids, glycosides, saponins, phytosterols, andtriterpenoids.The inhibitory effect was determined by Cup plate method, by the measurement of zone of inhibition.The outcome was the leavesextract consisted of active phytoconstituents which were phenols, glycoside, sugar and avanoid due to which the extract showed signicant zoneof inhibition,by which property of antibacterial and antifungal was listed. It can be concluded that the leaves of Pithecellobium dulce consist ofactive phytoconstituents which can be used as potent antimicrobials