Open AccessTHERAPEUTIC ROLE OF AZADIRACHTA INDICA LEAF EXTRACT AGAINST UTI (URINARY TRACT INFECTION)
Author(s) -
Kapil Singh
Publication year - 2020
Publication title -
global journal for research analysis
Language(s) - English
DOI - 10.36106/gjra/1704956
Subject(s) - azadirachta , proteus mirabilis , antimicrobial , agar , silver nanoparticle , agar diffusion test , escherichia coli , nutrient agar , minimum inhibitory concentration , antibacterial activity , traditional medicine , chemistry , microbiology and biotechnology , biology , bacteria , medicine , materials science , nanoparticle , biochemistry , nanotechnology , genetics , gene
Azadirachta indica is a multipurpose tree with multiple health benefits. Different parts of the plant are shown to exhibit antimicrobial effects against a wide variety of micro organisms. In the present study we investigate the antibacterial activity of Azadirachta indica extract against UTI (urinary tract infection) causing bacteria. Silver nanoparticles could be synthesized using the leaves of Azadirachta indica as reducing agent. The resulting silver nanoparticles were characterized using UV-Vis spectrophotometer, X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), and Fourier Transformations Infra Red (FTIR) spectroscopic analysis. The antibacterial activity was assayed by Agar well diffusion method using 20µl each of sterile Nutrient Agar (NA) (Hi-Media) and Potato-Dextrose Agar (PDA). The resulting silver nanoparticles exhibited best inhibitory activities against Escherichia coli and Proteus mirabilis which are main causing agents for UTI.